Antibacterial activity of plants 719 effective extracts are considered, e. In vitro antiplasmodial activity the plant extracts were tested in triplicate against chloroquine sensitive cqs strain of p. The proliferation and resistance of microorganisms area serious threat against humankind and the search for new therapeutics is needed. This is the first scientific report to document significant antiplasmodial activity of extracts from t. From the studies conducted on the plant until now, it is known that the methanolic root extract of the plant has schizonticidal activity as high as 84. Various phytotoxins released by necrotropic plant pathogenic fungi may inhibit the plantlike metabolic pathways in the apicoplast, a chloroplastlike organelle, essential for the survival of apicomplexan parasites, such as plasmodium which cause malaria in humans. European scientific journal april 2016 edition vol.
Sixtynine extracts from 11 plant species were prepared and screened for in vitro activity against p. The structures of isolated compounds were elucidated using chemical and spectroscopic methods. Drugs and plant extracts were then placed in 96well flat bottom tissue culture grade plates. In vitro antiplasmodial and cytotoxic activities of. The present study investigates the antimalarial activity of the methanolic extracts. Plant extracts showed moderate to good antiparasitic effects. Antimalarial and antiplasmodial activity papaya leaves are made into tea as a treatment for malaria. The current study was undertaken to evaluate the antiplasmodial activity and toxicity of the organic extracts of cassia alata. Okundoperoxide, a bicyclic cyclofarnesylsesquiterpene. Results in vitro antiplasmodial activity and cytotoxicity of aqueous extracts all five aqueous extracts showed activity against the c h l o r o q u i n e s e n s i t i v e 3 d 7 p.
The 50% inhibitory concentration ic50 values of the crude aqueous extracts were found to be 2. In vitro and in vivo antimalarial activity of plants from. In the in vivo evaluation of the antiplasmodial activity of the plant extracts was employed against. All extracts and pure compounds were tested for antiplasmodial activity in which the hexane bark extract showed highest activity with ic 50 0. Antiplasmodial activity of vernonia adoensis aqueous, methanol. The plant leaves were successively extracted into three 3 extract forms aqueous extract, ethanolic extract and hydroethanolic 50. Antimalarials from plant pathogenic fungi by mallika. Selected plants being used in indian traditional medicine to treat malaria and malarialike symptoms plant name, family, part used common name method of preparation referencs adhatoda zeylanica medic. Evaluation of the antiplasmodial activity and lethality of. In vitro antiplasmodial activity of extracts from cassia fistula tissues.
Antiplasmodial activity of the ethnobotanical plant. The antiplasmodial, antitrypanosomal and antileishmanial activity of 25 plant extracts obtained from seven tanzanian medicinal plants. Preliminary evaluation of selected medicinal plants for antiplasmodial activity 40 table 3. Nmrbased metabolomic study of medicinal plants used against malaria and the isolation of bioactive alkaloids. Promising antiplasmodial activity was found in the extracts from two plants, phyllanthus emblica leaf 50% inhibitory concentration ic 3d7. Antioxidant activity of the plant extracts were characterized by using dpph free radical scavenging method. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. The antiplasmodial activity of extracts of these plants was evaluated in vitro using the multiresistant strain w2 of plasmodium falciparum. Evaluation of antiplasmodial activity of extracts and constituents. In vitro antiplasmodial activity of aqueous and organic extracts from moringa ovalifolia was measured using parasitaemia posttreatment with plant extracts as well as the ic50 values. But all the mice treated with cq survived for 3 months additional file 1. Continuous in vitro cultures of asexual erythrocytes stages of p. Pdf antiplasmodial activity and phytochemical analysis of extracts.
Moreover, all plant extracts were found to have in vitro antiplasmodial activity. The antimalarial potential of aqueous leaf extracts of cymbopogon citratus was investigated in this present study. In this study the antiplasmodial effect of the leaf crude extract of v. Summary the in vitro antiplasmodial activities of 14 plant species traditionally used in central america for the treatment of malaria or fever were evaluated. Evaluation of antimalarial activity of hydromethanolic crude extract a. Antiplasmodial activity of methanol leaf extract of citrus. The in vitro antiplasmodial activity and cytotoxicity of extracts from jatropha tanjorensis leaves was evaluated. In vitro antiplasmodial activity and cytotoxicity of. The tannins extracts showed moderate antiplasmodial activity on both p.
Antiplasmodial properties of some malaysian medicinal plants. In nigeria, malaria is a major public health problem and there are high cost of the effective antimalarial drugs, poor quality drugs and increased emergence of plasmodial resistance. The results of antimicrobial activity showed that water and ethanol crude extracts were active on all the strains of pathogens tested at different concentrations, with ethanol extracts exerting more activity. Pdf in vitro antimalarial activity of medicinal plant extracts against. The two most active extracts that produced 100% inhibition of the parasite growth at concentration of plant material. The results showed antioxidant activity using dpph were found to be increased in a concentration dependent. The data revealed that significant reduction in growth of fungal strain f. The plant leaves were successively extracted into three 3 extract forms including aqueous extract, ethanolic extract and hydroethanolic 50.
Annickia enantia kummeriae annonaceae, artemisia annua asteraceae, pseudospondias microcarpa anacardiaceae, drypetes natalensis euphorbiaceae, acridocarpus chloropterus malpighiaceae, maytenus senegalensis celastraceae and. Antiplasmodial potential of medicinal plant extracts from. The methanolic leaf extract of ricinus communis is a low toxic plant with antiplasmodial activity which can be exploited as a potential source of new antimalarial drug to combat malaria. In vitro antiplasmodial activity and toxicity assessment of plant. Herein, the in vitro and in vivo antiplasmodial activity and cytotoxicity of medicinal plant extracts were investigated. Data analysis using twoway anova to determine the significant interactions between plant extracts and plasmodic growth. For quality control, high performance thin layer chromatography hptlc was performed on extracts. Bacterial diversity and community structure in typical plant rhizosphere. Antiplasmodial activity of extracts of tridax procumbens and phyllanthus amarus in in vitro plasmodium falciparum culture systems. The plant extracts analysed in this study presented an average antiplasmodial activity 58%.
In vitro assessment of the antiplasmodial activity of three plants. Evaluation of antiplasmodial activity of medicinal plants. Among the various extracts, the lowest 50% inhibitory concentrations ic50 of 24. The selected plants, acokanthera oppositifolia lam apocynaceae is a species encountered in eastern. In vitro antiplasmodial activity and phytochemicals. The plant extract was prepared by maceration in methanol. The in vitro antiplasmodial activity of seven etoh extracts and twenty fractions from the partition of the initial ethanolic extracts from seven african medicinal plants used in the democratic republic of congo dr congo for the treatment of malaria was evaluated. John oludele olanlokun, oluwole moses david, anthony jide afolayan. Antifungal activity of six botanical extracts was assayed and the effect of plant extracts on the growth of fungal strain f. The antiplasmodial activity of the extracts and pure compounds was evaluated using the standard peters fourday suppressive method. In vitro antiplasmodial activity of extracts and fractions. In vivo and in vitro antimalarial activity of vernonia. With regard to the ethanolic extract a, a high antiplasmodial activity ic 50 pdf antiplasmodial activity of plant extracts used in west african traditional medicine five plants originating from ivory coast were selected after an ethnobotanical survey, alchornea. The extracts of experimental plants were evaluated for their antiplasmodial activity against 3d7 and indo strains of p.
The antiplasmodial activities were carried out using the pldh assay to plasmodium falciparum d10 strain sensitive strain while the cytotoxic activities were carried out towards madindarby bovine kidney mdbk cells using mtt assay. Pdf in vitro antimalarial activity of medicinal plant. In vitro antiplasmodial activity and prophylactic potentials of extract. Antiplasmodial compounds from leaves of dodonaea angustifolia. The emergence and spread of plasmodium falciparum with resistance to chloroquine cq, the safest and cheapest antimalarial drug, coupled with the increasing cost of alternative drugs especially in developing countries have necessitated the urgent need to tap the potential of plants for novel antimalarials. The use of plant extracts with known antimicrobial properties, can be of great significance in therapeutic treatments. In previous study on antimalarial plants from mali 31 crude extracts of a. Seven of the eleven extracts exhibited significant antiplasmodial activity ic 50 edulis, c. Also, the methanol plant extracts were more potent than their corresponding aqueous extracts. In vitro antiplasmodial assays on plasmodium falciparum in human red blood cell culture. The purpose of this study was to discover antimalarial compounds from necrotropic plant pathogenic fungi. The methanolic extract phyllanthus niruri stone breaker showed the maximum activity against staphylococcus sp.
Quantitative assessment of antiplasmodial activity in. In vitro antiplasmodial activity of central american. Further studies are warranted to explore its efficacies and lead compounds of these three plant extracts for the. Antiplasmodial activity of some medicinal plants used in. Antimicrobial activity of selected medicinal plants. Evaluation of the antiplasmodial activity of extracts of. Thus, it is worth documenting the ethnobotanical information and testing the antiplasmodial activity of plant extracts. M as antiplasmodial activity when tested in vitro against plasmodium falciparum. Thus, there is a need for alternative source of medicine in malaria treatment and prevention. Promising antiplasmodial activity was found in the extracts from two plants, phyllanthus emblica leaf. Six methanol meoh extracts and one chloroform extract showed in vitro antiplasmodial activity against the d6 plasmodium falciparum strain, while only three.
Antiplasmodial activity and phytochemical analysis of extracts from selected ugandan medicinal plants. Preliminary evaluation of selected medicinal plants for. The plants are usually used to treat the malaria disease, and the people commonly use the extract of this plants for this purpose 5. Antiplasmodial, antitrypanosomal, antileishmanial and. Antibacterial activity of alkaloid extracts from ochrosia. Antiplasmodial activity was assessed 1 corresponding author. This suggests that leaves should be preferred over the whole plant and extraction should be made in ethyl acetate for enriching antiplasmodial molecules. Data analysis using twoway anova to determine the significant interactions between plant extracts and. The results of the 12 fractions of three of the bioactive plant extracts in the in vitro antiplasmodial assay. Antioxidant and in vitro cytotoxic activity of extracts of.
In sudan, out of 21 compounds isolated from 9 medicinal plants used in traditional medicine, only gedunin and quercetin showed ic50 of 1. The in vitro antiplasmodial activity of seven etoh extracts and twenty fractions from the partition of the initial ethanolic extracts from seven african medicinal plants used in the democratic. The present report describes the antiplasmodial and anticancer activities of samples isolated from the methanol extract of albizia zygia mimosaseae. Plant extracts were correspondingly subjected to an antiproliferative bioassay against mammalian skeletal myoblast cells. Other related study reported that screening of the appropriate extracts of ochrosia maculata elicited. Antiplasmodial activity and toxicity of crude extracts.
The in vitro cytotoxicity assay of the 6 plant crude extracts using kb cells indicated that t. Antiplasmodial activity of vernonia adoensis aqueous. In vitro antiplasmodial activities and synergistic. Lipophilic extracts of piper hispidum, siparuna andina, s. Antiplasmodial activity of methanol leaf extract of citrus aurantifolia christm swingle. Antiplasmodial activities of crude extract and solvent fractions were.
The cytotoxic activity of the extracts of cocculus hirsutus on mcf7 cells was investigated in vitro through mtt assay. The results from the in vitro antiplasmodial testing of 7 etoh extracts and 20 fractions from the partition of their respective initial etoh extracts are presented in table 2. Antiplasmodial activity of selected medicinal plants. Facilitate digestion the leaves of the papaya plants contain. The plant extracts were obtained from forest research institute malaysia frim. All plant samples displayed various antiplasmodial activity. Nmrbased metabolomic study of medicinal plants used. Both plant extracts also demonstrated antiplasmodial activity against the chloroquine resistant plasmodial parasites.
A preliminary screening of potentially antimalarial plants. Antiplasmodial activity of medicinal plant preparations. Antiplasmodial activity of aqueous leaf extract of. Larvicidal, antipyretic and antiplasmodial activity of. Thus, the present study aims at evaluating the invivo antiplasmodial activity of solvent fractions of methanolic root extract of. In vitro and in vivo antiplasmodial activities of gynura. In vitro antiplasmodial activity of dysoxylum caulostachyum miq. This study revealed for the first time the antiplasmodial activity of the plant c. In vitro antimalarial activity of medicinal plant extracts against plasmodium falciparum. Various extracts obtained from 19 plants collected in kenya were tested for antiplasmodial activity.
Pdf antiplasmodial activity and phytochemical analysis. Plant extract exhibited no signs of toxicity on mice at a dose of 5000. Evaluation of the antiplasmodial properties of namibian. Cytotoxycity and antiplasmodial activity of phenolic. Of these, extracts from artabotrys crassifolius, pericampylus glacus and leuconotis eugeniifolia showed promising antiplasmodial activities at ic 50 of 15.